Effects of Oleuropein on Epirubicin and Cyclophosphamide Combination Treatment in Rats

Objectives Oleuropein is the main bioactive polyphenolic compound in olive leaves, olive, and oil. Its anticancer, antioxidant, antiinflammatory effects have been proven through several vitro vivo studies. This study aimed to explore of oleuropein on cyclophosphamideand epirubicin-induced toxicity female rats. Materials Methods Seven groups containing eight rats each group were formed. Four cycles 16 mg/kg/week cyclophosphamide 2.5 epirubicin administered intraperitoneal injection. (150 mg/kg/week) was simultaneously applied via oral gavage. The examined with hemogram tests whole blood samples biochemical analysis serum samples. Interleukin-6 (IL-6) tumor necrosis factor-α (TNF-α) analyzed enzyme-linked immunosorbent assay. Subsequently, a comet assay performed using lymphocyte DNA. levels oxidant [i.e., malondialdehyde (MDA)] antioxidants catalase (CAT), glutathione (GSH), superoxide dismutase (SOD)] measured heart, kidney, liver tissues. Results could reduce DNA damage TNF-α IL-6 levels. It also ameliorated some parameters that deteriorated due antineoplastic drugs. increased amounts (GSH, SOD, CAT) reduced level MDA Conclusion might be beneficial agent against caused by combination treatment epirubicin. Further studies should demonstrate protective induced-toxicity precisely.